Journal: Pulmonary Circulation

Article Title: Transmembrane protein 16A/anoctamin 1 inhibitor T16A inh -A01 reversed monocrotaline-induced rat pulmonary arterial hypertension

doi: 10.1177/2045894020946670

Figure Lengend Snippet: Upregulation of TMEM16A expression in the PAs of PAH-modeling rats and its correlation with PCNA and WA%. Representative Western blots are shown. Pulmonary arterial tissues were obtained for Western blots from rats treated with MCT for 0 (control), 1 (1W), 2 (2W), 3 (3W), and 4 weeks (4W). Representative Western blot band of TMEM16A (a), PCNA (b), P-ERK1/2 (c) of pulmonary arterial tissues from rats treated with MCT for 0 (control), 1 (1W), 2 (2W), 3 (3W), and 4 weeks (4W). (d) to (f) Analytic data of TMEM16A, PCNA, P-ERK1/2 protein expression. (g) TMEM16A mRNA expression of pulmonary arterial tissues from rats treated with MCT for 0 (control), 1 (1W), 2 (2W), 3 (3W), and 4 weeks (4W). **: P < 0.01 versus control. n = 6 for each group. ERK1/2: extracellular regulated protein kinases; NS: not significant; PCNA: proliferating cell nuclear antigen; TMEM16A: transmembrane protein 16A.

Article Snippet: The proteins were separated on the gel and then transferred onto polyvinylidene difluoride membranes, which were blocked with 5% skimmed milk for 2 h and immunoblotted with a primary antibody against TMEM16A (Abcam, Cambridge, UK) at a dilution of 1:1000, a PCNA (Boster, CA, USA) at a dilution of 1:250, or an antibody against β-actin (Santa Cruz Biotechnology, Dallas, TX, US) at a dilution of 1:1000.

Techniques: Expressing, Western Blot, Control

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Flavokawain A alleviates the progression of mouse osteoarthritis: An in vitro and in vivo study

doi: 10.3389/fbioe.2022.1071776

Figure Lengend Snippet: Protective effects of FKA against IL-1β-induced inflammation and ECM destruction in mouse chondrocytes. Chondrocyte cell treatment with 5 ng/ml of IL-1β only or with FKA (20 and 40 μM) for a period of 24 h. Western blot analysis of inflammatory cytokines (COX2 and iNOS), catabolic (ADAMTS5, MMP3, and MMP13), and anabolic markers (Col2, Aggrecan, and Sox9) (A,C and E) . Quantitative analysis of protein expression (B,D and F) . (G and H) 5 ng/ml of IL-1β was added for treating the cells for 24 h in the absence or presence of 40 µM FKA. Confocal microscopy showing the expression of MMP13 and Aggrecan in immunofluorescence experiments (scale bar 10 μm). (I) Relative quantification of fluorescence intensity. The values are presented as means ± SD ( n = 3). # p < 0.05 vs the control group; * p < 0.05 and ** p < 0.01 vs the IL-1β group.

Article Snippet: ADAMTS5 primary antibody, secondary antibody, phosphate-buffered saline (PBS), trypsin, collagenase type II, the CCK8 assay kit, bovine serum albumin (BSA), and protein extraction kit were ordered and acquired from Boster Biological Technology (Wuhan, Hubei, China).

Techniques: Western Blot, Expressing, Confocal Microscopy, Immunofluorescence, Quantitative Proteomics, Fluorescence, Control